Du test
- Confirmation for Rhesus-negative.
Procedure:
When the blood sample did not form agglutination with the Anti-D reagent, indicates that the sample is Rhesus-negative.
When the blood sample did not form agglutination with the Anti-D reagent, indicates that the sample is Rhesus-negative.
- Incubate the sample solution in water bath for 15 minutes.
- Spin the test tube at 3000 rpm for 15 seconds.
- If the result remain negative, wash three times at 3000 rpm for 1 minute each.
- Dispose the supernatant and add 2 drops of AHG.
- Spin the test tube at 3000 rpm for 15 seconds.
- Look for any agglutination under microscope.
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