Wednesday, October 21, 2015

Du test.

Du test 
  • Confirmation for Rhesus-negative.

Procedure:

When the blood sample did not form agglutination with the Anti-D reagent, indicates that the sample is Rhesus-negative. 
  1. Incubate the sample solution in water bath for 15 minutes.
  2. Spin the test tube at 3000 rpm for 15 seconds.
  3. If the result remain negative, wash three times at 3000 rpm for 1 minute each.
  4. Dispose the supernatant and add 2 drops of AHG.
  5. Spin the test tube at 3000 rpm for 15 seconds.
  6. Look for any agglutination under microscope.

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