Blood Smear

Blood smear/ Blood film.

How to do a Blood Smear?

1. Place a small drop of blood at one end of the smear to be made on the slide.
2. Position the spreader (we use cover slip here) at 45° angle and backing into the drop of blood.

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Differentiation of gender based on Blood Smear.

Female and male  have same amount of neutrophils bearing appendages. ( A to D form )

However, female has more A-form (drumstick) appendages while male has more C-form (tag) appendages due to higher androgen level in men.

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References:

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781649/