- Hemoglobin A1c test, is an important in vitro diagnostic test for quantitative determination of glycated hemoglobin in human whole blood.
- It aids in the diagnosis of diabetes mellitus.
- A1c (glycated hemoglobin) is formed in the blood when glucose attached on the hemolobin.
Procedure:
(*Everyday start with QC)
- Shake tube and record patient's data in the book.
- Take cartridge ( Make it stand on the table, because inside have solution)
(*Do not touch or leave any fingerprint on the bottom part of the cartridge) - Press the "patient" icon & insert the cartridge
- Insert user ID and patient MRN.
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| Cartridge |
 |
| AS100 analyser. |
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| Cartridge ( Before ) Have to use the capillary tube to obtain the required amount of blood for testing. |
Cartridge ( After )
Cartridge ( After ) which the capillary tube is shown **the blood sample was replaced with blue reagent.**
HbA1c levels:
- Normal: 4% & 5.6%
- Abnormal: 5.7% & 6.4% (39 & 47 nmol/mol)
Limitations:
(Cannot diagnose)
- Patient with a hemoglobinopathy
- Patient with abnormal red cell turnover
- Patient with iron deficiency & hemolytic anemia,...
- Patient that have received a blood transfusion/ cancer chemotheraphy within the past 3 weeks
- Cannot use coagulated/ hemolysed sample
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