STart analyser / PT, aPPT & INR

Specimen: Patient's blood within the Sodium Citrate tube ( Blue top )

What is INR?
INR is known as the international normalized ratio.
It is used to monitor patient who has been treated with an anticoagulant, warfarin for blood thinning purpose.
It is a calculation for the appropriate drug dosage for patient based on the result of PT.





*normal=control
*ISI = ISI value for the analytical system being used.






Definition for PT test?
PT test is often used along with APTT to investigate on any bleeding or clotting disorder.

The PT evaluates the coagulation factor VII, X, V, II and I ( Fibrinogen ).
The APTT evaluates coagulation factor XII, XI, IX, VIII, X, V, II (Prothrombin), and I (Fibrinogen) , as well as PK (Prekallikrein) and HK (High Molecular Weigh Kininogen).

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STart analyzer

*The blue pointer show the pipette tips*
*The red pointer shows the finnpipette*

Steel ball inside the cuvette ( pointed )

Methodology:

PT

1. 50µl Plasma is inserted into a cuvette which containing a steel ball, and placed on the channel of the STart analyser.
2. Next, start the timer and incubate for 60sec.
3. After incubation, put the measuring test tube on the rack for the addition of reagent.
4. 100µl of Neoplastine CI plus can be released from the finnpipette only when the release button is pressed.

APTT

1. 50µl Plasma + 50µl CK Prest are inserted into a cuvette which containing a steel ball, and placed on the channel of the STart analyser.
2. Next, start the timer and incubate for 180sec.
3. After incubation, put the measuring test tube on the rack for the addition of reagent.
4. 50µl of CaCl2 (0.025 pre-warmed) reagent is added.