Urea Breath Test

Besides expose to the biochemistry analyser, I also have the chance to run the urea breath test using Urea breath test analyser ( Brand: Pytest ).

This test is used to monitor the treatment undergo by patient which suspected to be infected by Helicobacter pylori .
There are a few advantages using this test :

•  Cost effective
•  Shorter Turn around time
•  Not invasive

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Checklist

• Calibration
• Make sure there is no fingerprint on the Background sample ( BKG )
• Make sure there is no fingerprint on the 14C Reference Standard
• Check if there is air coming out from the vial tubing after the machine is switched on.
  

Methodology

Procedure 1:

1. Swallow a yellow pill (Urea) with one small cup of water and wait for 3 minutes.
2. Then drink another cup of water and wait for 7 minutes.
3. Blow the balloon.

Procedure 2: 

All the equipment needed for Urea Breath Test.

Add 2.5ml PYest Urea Breath Test reagent (Blue in color and a store in amber bottle)  into a bottle.

Insert the balloon tubing into the balloon and vial tubing into the bottle.

Remove the tubing when the solution turns colorless.

Add 10 ml Scintillation fluid into the bottle.

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Swirl it gently until the solution turns clear.

Insert the bottle into the hole of the analyzer.

Press the [ Count ] button to start the analysis