Mycoplasma pneumonia is associated with various non respiratory diseases such as meningitis, encephalitis, pancreatitis and arthritis. Culture of the organism can be used for the diagnosis of infection, however it will be difficult because it is insensitive and takes about 3 weeks for the result. For this reason, we only apply serological method for the diagnosis of M.pneumonia infection in our laboratory.
Principle of the test:
(Colloidal Gold Chromatography)
The Control region consist of HAMAs;
Test region consists of MP antigen;
Sample well consists of anti-human MP antibody.
If the sample containing MP antibody, it will form complexes with the anti-human MP antibody.
The complexes will then bind to the immobilized MP antigen and forms a red line at the test region while the unbounded complexes will be captured by the HAMAs at C region and form red line indicating the validity of the test kit.
Sample Preparation:
Fresh whole blood, plasma or serum is used as the cardiac proteins are relatively unstable.
Procedure:
- Add 1 drop of the sample (30μl) and 2 drops of buffer to the sample well of the test panel.
- Start the timer ( 15 minutes) *if after 20 minutes, the result will be invalid.*
Interpretation:
- Solely Control line --- Negative
- Solely Test line --- Invalid
- Both Control and Test line --- Positive
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