- It is a test that used to diagnose tuberculosis ( caused be Mycobacterium tuberculosis) in the patient and help in monitoring the effectiveness of treatment.
- AFB test may also be used to detect any infection caused by the Mycobacterium species (Acid fast bacilli).
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| The pink rod-shaped microorganism is Mycobacterium tuberculosis |
Procedure:
- Prepare a smear and fix.
- Flood the smear with ZN-carbol fuchsin stain for 5 minutes and gently heat it.
- Wash gently with running tap water.
- Decolorize with TB-decoloriser for 2~3minutes
- Wash gently with running tap water.
- Counter stain with methylene blue for 30~60 seconds.
- Wash gently with running tap water.
- Dry over gentle heat.
- Examine under 100x with immersion oil.
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