Important notes during streak plate.

Important Notes

1. Don't touch the wall using loop while streaking because the side wall of the agar plate is consider not sterile. ( except for Mueller Histon agar )
   
2. 
   
   
   
3. Blood Agar (BA) should be streaked first before MacConkey (MAC) agar to avoid contamination by the bile salt and crystal violet in MAC which used to inhibit the growth of Gram-positive organism.
   (Every microorganism can grow on BA)

   

   (Left) MAC agar  ; (Right) BA 

4. Function of every types of used agar should be studied in order to determine which types of organism can be cultured on it.
   
5. Sometimes when Urine FEME showed 3+ positive in the amount of bacteria, however there is no growth on the agar culture. This is because the bacteria observed under the microscope might be already dead or it could also be possible that there is mistake in the technique used to culture the plate.
   
6. Gram-positive and gram-negative organism should not present together in certain part of the body. If the culture shows this type of result, we must always correlate with the FBC ( Full Blood Count ) result to see whether there is any infection.