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Equipment:

6 types of reagent .

Procedure:

1. Add 8000μl of saline in a container.
2. Withdraw 100μl of the saline and replace it with the same amount of patient's serum. 
3. Add 1000μl of the diluted sample into 6 tests tubes which containing 6 different types of  reagents in it. (1 drop in each)
4. Incubate in water bath for 5 hours.

Result Interpretation:

• Positive result will shown in the sedimentation of the colored reagent.